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A resting cell system for efrotomycin biosynthesis.

J B Nielsen¹, L Kaplan

¹Merck & Co., Inc., NJ 07065-0900.

The Journal of Antibiotics

|June 1, 1989

Summary

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Nocardia lactamdurans produces efrotomycin, an antibiotic used as a growth permittant. A new resting cell system aids in studying efrotomycin biosynthesis and glycosylation.

Area of Science:

Microbiology
Biochemistry
Biotechnology

Background:

Efrotomycin is a modified polyketide antibiotic produced by Nocardia lactamdurans.
It is utilized as a growth permittant in the animal industry.
Biosynthetic studies were limited prior to the development of a new system.

Purpose of the Study:

To develop a resting cell system for studying efrotomycin biosynthesis.
To investigate factors influencing efrotomycin production and utilization of carbon sources.
To examine the glycosylation pathway of efrotomycin synthesis.

Main Methods:

Development of a resting cell system using Nocardia lactamdurans.
Supplementation with carbon sources (optimally glucose) to washed cells.

Related Experiment Videos

Utilizing protein synthesis dependent and independent systems for precursor incorporation and inhibitor studies.

Employing a system from an efrotomycin non-producer to study aglycone glycosylation.

Main Results:

The resting cell system produced up to 700 mg/ml efrotomycin in 60 hours.
No carbon or nitrogen repression was observed.
Productivity decreased with cell age but showed less dependence on protein synthesis.
The glycosylation of aurodox to efrotomycin via 6'-deoxyallosyl aurodox was examined.

Conclusions:

The developed resting cell system is effective for efrotomycin biosynthesis studies.
Efrotomycin production is influenced by cell age and carbon source availability.
The study elucidated key steps in the efrotomycin glycosylation pathway.