Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

457
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
457
Amino Acid Catabolism01:18

Amino Acid Catabolism

1.5K
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
1.5K
Sulfur Assimilation01:20

Sulfur Assimilation

477
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
477

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vancomycin population pharmacokinetics for adult patients with sepsis or septic shock: are current dosing regimens sufficient?

European journal of clinical pharmacology·2019
Same author

Clinicians' prescribing practices for bariatric surgery patients: is there an issue?

Journal of clinical pharmacy and therapeutics·2014
Same author

Intestinal mast cells and eosinophils in relation to Strongyloides ratti adult expulsion from the small and large intestines of rats.

Parasitology·2013
Same author

Optineurin is potentially associated with TDP-43 and involved in the pathogenesis of inclusion body myositis.

Neuropathology and applied neurobiology·2012
Same author

Faecal chitinase 3-like-1: a novel biomarker of disease activity in paediatric inflammatory bowel disease.

Alimentary pharmacology & therapeutics·2011
Same author

Strongyloides ratti: transplantation of adults recovered from the small intestine at different days after infection into the colon of naive and infection-primed Wistar rats, and the effect of antioxidant treatment on large intestinal parasitism.

Parasitology·2011

Related Experiment Video

Updated: Mar 18, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

9.1K

Glutamate Overproduction in Corynebacterium glutamicum Triggered by a Decrease in the Level of a Complex Comprising

E Kimura1, C Yagoshi1, Y Kawahara1

  • 1a Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

Bioscience, Biotechnology, and Biochemistry
|July 6, 2016
PubMed
Summary

A decrease in DtsR protein levels triggers glutamate overproduction in Corynebacterium glutamicum by reducing 2-oxoglutarate dehydrogenase complex activity. This finding offers insights into metabolic regulation for enhanced amino acid synthesis.

Keywords:
Corynebacterium glutamicumDtsRTween 40biotinglutamate overproduction

More Related Videos

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.7K
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.8K

Related Experiment Videos

Last Updated: Mar 18, 2026

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
06:45

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

9.1K
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.7K
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
06:24

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

10.8K

Area of Science:

  • Microbiology
  • Biochemistry
  • Metabolic Engineering

Background:

  • Glutamate overproduction in Corynebacterium glutamicum is a key industrial process.
  • This overproduction can be induced by various factors, including Tween 40, biotin limitation, or penicillin.
  • The dtsR gene plays a role in regulating this process.

Purpose of the Study:

  • To investigate the role of the dtsR gene in regulating glutamate overproduction.
  • To elucidate the mechanism by which DtsR influences glutamate synthesis.
  • To understand the relationship between DtsR levels, Tween 40, biotin limitation, and enzyme activity.

Main Methods:

  • Gene disruption and overexpression of the dtsR gene in Corynebacterium glutamicum.
  • Analysis of glutamate production levels under different conditions.
  • Measurement of 2-oxoglutarate dehydrogenase complex (ODHC) activity.
  • Monitoring DtsR protein levels in wild-type strains.

Main Results:

  • Disruption of dtsR led to constitutive glutamate overproduction.
  • Overexpression of dtsR inhibited glutamate overproduction induction.
  • DtsR protein levels decreased under inducing conditions (Tween 40, biotin limitation).
  • Inducing conditions and dtsR disruption reduced ODHC activity.

Conclusions:

  • A decrease in DtsR levels, or a complex containing it, initiates glutamate overproduction.
  • This initiation is mediated by the downregulation of 2-oxoglutarate dehydrogenase complex (ODHC) activity.
  • The findings reveal a novel regulatory mechanism for glutamate biosynthesis in C. glutamicum.