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Development of a multiplex PCR assay for rapid virulence factor profiling of extraintestinal pathogenic Escherichia
Toru Ojima1, Kaori Hirano1, Kohei Honda1
1Yamagata Prefecture Central Livestock Hygiene Service Center, 736 Urushiyama, Yamagata 990-2161, Japan.
Journal of Microbiological Methods
|July 7, 2016
Abstract:
Virulence factor (VF) profiling is important for the control of extraintestinal pathogenic Escherichia coli (ExPEC) infection because VF prevalence is highly variable. We analyzed the VF profile of ExPEC isolated from cattle in Yamagata prefecture, Japan, 2000-2015 and developed a rapid VF profiling method using a multiplex PCR assay.
