Spreading depolarization triggered by elevated potassium is weak or absent in the rodent lower brain

We examined in live coronal slices from rat and mouse which brain regions generate potassium-triggered spreading depolarization (SD_Kt). This technique simulates cortical spreading depression, which underlies migraine aura in the intact brain. An SD_Kt episode was evoked by increasing bath [K⁺]_o and recorded as a propagating front of elevated light transmittance representing transient neuronal swelling in gray matter of neocortex, hippocampus, striatum, and thalamus. In contrast, SD_Kt was not imaged in hypothalamic nuclei or brainstem with exception of those nuclei near the dorsal brainstem surface. In rat slices, single neurons were whole-cell current clamped during SD_Kt. "Higher" neurons depolarized to near zero millivolts indicating SD_Kt generation. In contrast, seven types of neurons in hypothalamus and brainstem only slowly depolarized without generating SD_Kt, supporting our imaging findings. Therefore, SD_Kt is not a default of CNS neurons but rather displays a region-specific susceptibility, similar to anoxic depolarization, which we have proposed is correlated with a region's vulnerability to traumatic brain injury. In the higher brain, SD_Kt may be a vestigial spreading depolarization that originally evolved to shut down and vasoconstrict gray matter regions more exposed to impact and contusion.