Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Agarose Gel Electrophoresis

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An Alternate Process for the Solid-Phase Synthesis and Solid-Phase Purification of Synthetic Nucleic Acid Sequences.

Current protocols·2023

Same author

Synthesis of an Atom-Specifically <sup>2</sup> H/<sup>13</sup> C-Labeled Uridine Ribonucleoside Phosphoramidite.

Current protocols·2022

Same author

Synthesis of Unmodified Oligonucleotides.

Current protocols in nucleic acid chemistry·2022

Same author

Use of Arabinonucleosides for Development and Implementation of a Novel 2'-O-Protecting Group for Efficient Solid-Phase Synthesis and 2'-O-Deprotection of RNA Sequences.

Current protocols·2022

Same author

An Improved PEG-Linked Solid Support for Minimizing Process-Related Impurities During Solid-Phase Synthesis of DNA and RNA Sequences.

Current protocols·2021

Same author

Innovative 2'-<i>O</i>-Imino-2-propanoate-Protecting Group for Effective Solid-Phase Synthesis and 2'-<i>O</i>-Deprotection of RNA Sequences.

The Journal of organic chemistry·2021

Search research articles

Related Experiment Video

Updated: Mar 18, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Solid-Phase Purification of Synthetic DNA Sequences.

Andrzej Grajkowski¹, Jacek Cieslak¹, Serge L Beaucage¹

¹Laboratory of Biological Chemistry, Division of Biotechnology Review and Research IV, Center for Drug Evaluation and Research, Food and Drug Administration , 10903 New Hampshire Avenue, Silver Spring, Maryland 20933, United States.

The Journal of Organic Chemistry

|July 8, 2016

Summary

A novel purification method efficiently isolates synthetic DNA sequences using a silica gel functionalization technique. This high-throughput process achieves over 98% purity for DNA, crucial for synthetic biology and therapeutics.

More Related Videos

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

Related Experiment Videos

Last Updated: Mar 18, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

Published on: January 5, 2011

Area of Science:

Biochemistry
Organic Chemistry
Materials Science

Background:

High-throughput DNA synthesis is established for synthetic biology and nucleic acid therapeutics.
Efficient purification methods for synthetic DNA sequences remain underdeveloped.

Purpose of the Study:

To develop a high-throughput purification process for synthetic DNA sequences.
To enable large-scale production of pure synthetic nucleic acids for various applications.

Main Methods:

Functionalizing aminopropylated silica gel with aminooxyalkyl groups.
Capturing DNA sequences with a 5'-siloxyl ether linker and keto function via oximation.
Incorporating functionalized deoxyribonucleoside phosphoramidites into DNA synthesis.
Releasing purified DNA using tetra-n-butylammonium fluoride in DMSO.

Main Results:

Functionalized phosphoramidites prepared in 75-83% yield.
Near-quantitative capture of deprotected DNA sequences.
Achieved >98% purity for released DNA sequences.
Demonstrated scalability and high-throughput capability.

Conclusions:

Developed an efficient, scalable, high-throughput DNA purification method.
The oximation-based capture strategy yields highly pure synthetic DNA.
This method supports advancements in synthetic biology and nucleic acid therapeutics.