Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tuning the Crystallite Size, Shape, and Magnetic Properties of Fe<sub>3</sub>O<sub>4</sub> Nanoparticles Using Annealing.

Materials (Basel, Switzerland)·2026

Same author

Emergence of magnetic monopole-like behavior in iron oxide nanoparticles grafted with chiral brushes: a chiral induced spin selectivity manifestation.

Materials horizons·2026

Same author

Interplay Between Exfoliation and Functionalization Strategies for Group VI Layered Transition Metal Dichalcogenide Dispersions.

Nanomaterials (Basel, Switzerland)·2026

Same author

Dynamic monitoring of bone homeostasis by dual fluorescent imaging of osteoblast and osteoclast activity.

Acta biomaterialia·2026

Same author

Siloxane-Terminated Self-Assembled Monolayers for Mimicking Nanoscale Hydrophobic Polydimethylsiloxane Surfaces.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Stabilizing the Localized Surface Plasmon Resonance (LSPR) of Citrate-Synthesized Metal Nanoparticles in Organic Solvents.

Materials (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Mar 18, 2026

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Published on: February 10, 2023

Robust Maleimide-Functionalized Gold Surfaces and Nanoparticles Generated Using Custom-Designed Bidentate Adsorbates.

Chul Soon Park¹, Han Ju Lee¹, Andrew C Jamison¹

¹Department of Chemistry and the Texas Center for Superconductivity, University of Houston , 4800 Calhoun Road, Houston, Texas 77204-5003, United States.

Langmuir : the ACS Journal of Surfaces and Colloids

|July 8, 2016

Summary

Custom alkanethioacetate ligands enable robust attachment of molecules to gold nanostructures. Ligands with oligo(ethylene glycol) (OEG) and chelating dithioacetate groups enhance nanoparticle stability in solution and resist displacement.

More Related Videos

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Published on: July 2, 2019

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

Related Experiment Videos

Last Updated: Mar 18, 2026

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Published on: February 10, 2023

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Synthesis and Characterization of Amphiphilic Gold Nanoparticles

Published on: July 2, 2019

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

Area of Science:

Materials Science
Surface Chemistry
Nanotechnology

Background:

Developing stable gold nanostructures requires effective surface modification techniques.
Controlling the attachment and stability of adsorbates on gold surfaces is crucial for various applications.

Purpose of the Study:

To synthesize custom alkanethioacetate ligands for facile attachment of maleimide-terminated adsorbates to gold nanostructures.
To investigate the influence of ligand structure, including oligo(ethylene glycol) (OEG) moieties and dithioacetate groups, on monolayer formation and gold nanoparticle (AuNP) stability.

Main Methods:

Synthesis of mono- and dithioacetate ligands with varying alkyl spacers and OEG content.
Characterization of monolayers on gold substrates using X-ray photoelectron spectroscopy, FTIR-reflection-absorption spectroscopy, ellipsometry, and contact angle goniometry.
Preparation and stability assessment of gold nanoparticles (AuNPs) under extreme conditions (high salt, ligand exchange) using visual and spectroscopic methods.

Main Results:

Monolayer formation was solvent-dependent, with EtOH yielding higher packing density and THF providing better thiolate binding.
AuNPs functionalized with OEG-containing ligands showed enhanced colloidal stability in high salt concentrations.
AuNPs with dithioacetate ligands demonstrated improved resistance to ligand exchange, particularly against dithiothreitol (DTT).

Conclusions:

Chelating dithioacetate ligands incorporating OEG moieties provide the most effective combination of stability and solubility for gold nanostructures.
The chelate effect and OEG groups significantly enhance adsorbate attachment and colloidal stability in aqueous solutions.