Related Experiment Video
Updated: Mar 18, 2026
Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
Published on: May 16, 2014
Production of Biodegradable Polyester by a Transgenic Tobacco
H Nakashita1, Y Arai2, K Yoshioka1
1a The Institute of Physical and Chemical Research (RIKEN).
Abstract:
The acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha and the poly[(R)-(-)-3-hydroxyalkanoate] synthase gene (phaCAC) of Aeromonas caviae were introduced into tobacco plant by Agrobacterium mediated transformation method. The resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. GC-MS analysis of the chloroform extract of tobacco leaves indicated that the transgenic plant produced biodegradable polyester, poly-[(R)-(-)-3-hydroxybutyrate]. GPC analysis indicated that the number-average molecular weights (Mn) and polydispersity (Mw/Mn) were 32,000 and 1.90, respectively.
More Related Videos
Related Concept Videos
01:20Types of Step-Growth Polymers: Polyesters
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
02:50Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
00:53Transgenic Organisms
01:59Plant Breeding and Biotechnology