Related Experiment Video

Updated: Mar 18, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.2K

Investigation of GPCR allosterism and dimerization in single living cells using fluorescent ligands

Stehen Hill1

  • 1Univerisity of Nottingham, UK.

Springerplus
|July 8, 2016
PubMed
Abstract

No abstract available in PubMed .

Keywords:
Ligand bindingcooperativityfluorescence

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.8K
G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

10.1K

Related Experiment Videos

Last Updated: Mar 18, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.2K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.8K
G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

10.1K

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

Articles linked to this work by shared authors, journal, and citation graph.

In-silico combinatorial design and pharmacophore modeling of potent antimalarial 4-anilinoquinolines utilizing QSAR and computed descriptors.

SpringerPlus·2017

Erratum to: Implication of Paris Agreement in the context of long-term climate mitigation goals.

SpringerPlus·2017

Erratum to: Associations between adherence, depressive symptoms and health-related quality of life in young adults with cystic fibrosis.

SpringerPlus·2017

Erratum to: Numerical method to compute acoustic scattering effect of a moving source.

SpringerPlus·2017

Identifying appropriate protected areas for endangered fern species under climate change.

SpringerPlus·2017

An Algorithm to detect balancing of iterated line sigraph.

SpringerPlus·2017

A Retrospective Comparative Analysis of Sperm Count and Semen Parameters among White-Collar and Blue-Collar Workers: Influence of Occupational and Lifestyle Factors.

Journal of occupational and environmental medicine·2026

Live Imaging of Ions in Cells Infected with Chlamydia trachomatis.

Methods in molecular biology (Clifton, N.J.)·2026

Single-cell analysis of StkP activity reveals the temporal dynamics of DivIVA phosphorylation in Streptococcus pneumoniae.

mBio·2026

Broadband backscattering confocal microscopy enables label-free 3D live cell nanoscale sensitive imaging.

Science advances·2026

Chloride-dependent mechanisms contribute to urinary bladder smooth muscle contractility: Pharmacological and molecular evidence from rat bladder tissue.

Tzu chi medical journal·2026

Rad1-Rad10 uses different interfaces to interact with pathway-specific DNA repair factors.

Nucleic acids research·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us