Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

9.6K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
9.6K
The Extracellular Matrix01:42

The Extracellular Matrix

90.8K
Overview
90.8K
The Extracellular Matrix01:29

The Extracellular Matrix

13.1K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
13.1K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

3.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
3.7K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

3.6K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.6K
Anchoring Junctions01:03

Anchoring Junctions

5.5K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond birth control: how oral contraceptives impact skeletal muscle health: a systematic review and meta-analysis.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same author

Resolving differential vascular graft remodeling using longitudinal multiphoton tracking in a 3D culture platform.

bioRxiv : the preprint server for biology·2026
Same author

4-Hydroxybutyrate (4HB) released from poly-4-hydroxybutyrate scaffolds does not impact hallmark phenotypes of cancer in malignant or non-malignant breast cells.

Breast cancer research : BCR·2026
Same author

Extracellular Matrix Hydrogel Reduces Anastomotic Leaks in a Rodent Model of Rectal Anastomosis.

The Journal of surgical research·2025
Same author

Unlocking the regenerative properties of extraembryonic membrane-derived biomaterials in tissue engineering.

Acta biomaterialia·2025
Same author

Histomorphologic Outcomes of GalaFLEX Scaffold Used in Breast Surgery: Clinical Follow-up From 6 Weeks to 63 Months.

Aesthetic surgery journal·2025
Same journal

Taphonomic analysis at Liang Bua reveals the behavioral and technological capabilities of <i>Homo floresiensis</i>.

Science advances·2026
Same journal

Targeting granule initiation and amyloplast structure to create giant starch granules in wheat.

Science advances·2026
Same journal

A meta-analysis of carbon losses and gains from tropical moist forest degradation and regeneration.

Science advances·2026
Same journal

Ancient DNA reveals elite dynastic rule among Iron Age Eurasian Steppe nomads.

Science advances·2026
Same journal

Targeting astrocytic Dp71 attenuates BBB disruption after traumatic brain injury through WTAP-associated m<sup>6</sup>A regulation of MMP2.

Science advances·2026
Same journal

Pancreatic α cells are required for nutrient homeostasis by regulating dynamic β cell networks in islets.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

13.0K

Matrix-bound nanovesicles within ECM bioscaffolds.

Luai Huleihel1, George S Hussey1, Juan Diego Naranjo1

  • 1McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA.; Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA.

Science Advances
|July 8, 2016
PubMed
Summary
This summary is machine-generated.

Extracellular matrix scaffolds contain matrix-bound nanovesicles (MBVs) that influence cell behavior. These MBVs, a newly identified component of connective tissue, transfer microRNAs to regulate macrophage and neuroblastoma cell functions.

Keywords:
ExosomesExtracellular Matrix (ECM)Extracellular vesicles (EV)Matrix Bound Nano Vesicles (MBV)Microvesicles (MV)

More Related Videos

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.4K
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

9.2K

Related Experiment Videos

Last Updated: Mar 18, 2026

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

13.0K
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

4.4K
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
07:19

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Published on: September 7, 2018

9.2K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Extracellular matrix (ECM) scaffolds promote tissue regeneration via angiogenesis, stem cell recruitment, and anti-inflammatory responses.
  • Mechanisms underlying ECM scaffold-mediated constructive remodeling are not fully understood.
  • Extracellular vesicles (EVs) are known mediators of intercellular communication.

Purpose of the Study:

  • To identify and characterize matrix-bound nanovesicles (MBVs) within ECM scaffolds.
  • To investigate the functional role of MBVs in cellular processes.
  • To elucidate the contribution of MBVs to the therapeutic properties of ECM scaffolds.

Main Methods:

  • Enzymatic digestion of ECM scaffolds to isolate MBVs.
  • Characterization of MBV contents, including microRNA.
  • In vitro assays to assess MBV effects on macrophage activation and neuroblastoma cell differentiation.

Main Results:

  • MBVs were identified as integral components of ECM scaffolds, previously unreported in connective tissue.
  • MBVs were released from scaffolds upon enzymatic digestion, mirroring in vivo degradation.
  • MBVs contain microRNA that can alter macrophage phenotype and induce neuroblastoma cell differentiation.

Conclusions:

  • MBVs are a key functional component of ECM scaffolds, mediating their regenerative effects.
  • The presence of EVs within the ECM provides novel insights into tissue homeostasis regulation.
  • MBVs represent a potential therapeutic target for regenerative medicine and tissue engineering.