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Related Experiment Video

Updated: Mar 18, 2026

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Published on: May 21, 2020

In vitro osteocytic microdamage and viability quantification using a microloading platform.

S L York¹, P Sethu², M M Saunders¹

¹Department of Biomedical Engineering, The University of Akron, Akron, OH 44325, USA.

Medical Engineering & Physics

|July 9, 2016

Summary

This summary is machine-generated.

Osteocytes regulate bone remodeling. Lower mechanical strain increased osteocyte activity, while higher strain reduced it, demonstrating a method to induce microdamage for bone mechanotransduction studies.

Keywords:

Bone Remodeling Mechanotransduction Osteocyte

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A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

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Published on: February 24, 2023

Area of Science:

Biomedical Engineering
Cell Biology
Orthopedics

Background:

Bone remodeling involves osteoclast resorption and osteoblast formation, crucial for skeletal integrity.
Mechanical load significantly influences bone remodeling, with osteocytes hypothesized to play a regulatory role.
Mechanotransduction studies investigate cellular responses to mechanical stimuli, offering insights into bone cell regulation.

Purpose of the Study:

To design, fabricate, and characterize a novel mechanical loading platform for bone mechanotransduction research.
To investigate the role of osteocytes in response to variable mechanical strain.
To quantify cellular activity of osteocyte-like cells under a non-uniform strain field.

Main Methods:

Development of a mechanical loading platform applying out-of-plane distension to cell-seeded substrates.
Fabrication of an alphanumerically gridded polydimethylsiloxane well substrate for localized strain application.
Culturing and applying variable mechanical strains to MLO-Y4 osteocyte-like cells to assess cellular activity.

Main Results:

The platform successfully generated a non-uniform strain profile across the substrate.
Osteocyte-like MLO-Y4 cells exhibited increased activity in regions of lower mechanical strain.
Higher mechanical strains correlated with a reduction in cellular activity, indicating mechanically-induced microdamage.

Conclusions:

The developed microloading platform effectively exposes cells to mechanically-induced microdamage.
Osteocyte cellular activity is strain-dependent, with lower strains promoting activity and higher strains inhibiting it.
This platform facilitates further investigation into osteocyte mechanotransduction and its role in bone remodeling.