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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Dibenzo-α-pyrones from the endophytic fungus Alternaria sp. Samif01: isolation, structure elucidation, and their
Jin Tian1,2, Linyun Fu1, Zihao Zhang1
1a Department of Plant Pathology, College of Plant Protection , China Agricultural University , Beijing , China.
Abstract:
The EtOAc extract of the liquid fermentation of Alternaria sp. Samif01, an endophytic fungus obtained from Salvia miltiorrhiza Bunge, showed antibacterial activity against several tested bacterial pathogens. Fractionation of this extract led to the isolation of seven dibenzo-α-pyrones (1-7), including one new compound, 2-acetoxy-2-epi-altenuene (1) and one new natural product, 3-epi-dihydroaltenuene A (2). The structures of the new metabolites were elucidated by comprehensive analysis of the spectroscopic data including (1D, 2D) NMR and HRESIMS, while the absolute configuration of 1 was determined by TDDFT-ECD computation. Altenuisol (5), 4-hydroxyalternariol-9-methyl ether (6), and alternariol (7) showed inhibitory activities against the tested bacteria with minimum inhibitory concentration values in the range of 86.7-364.7 μM. A preliminary structure-antibacterial activity relationship was discussed. In addition, compounds 2, 5 and 6 displayed promising antioxidant effects using DPPH and hydroxyl radical assays. The cytotoxicity of the isolated compounds was evaluated as well.
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