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Updated: Mar 18, 2026
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Combining Imaging and Electrophysiology to Visualize and Record Spreading Depolarizations in Mice
Published on: October 4, 2024
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Ischemia-induced spreading depolarization in the retina
Anja I Srienc1, Kyle R Biesecker2, Angela M Shimoda3
1Graduate Program in Neuroscience, University of Minnesota, MN, USA Medical Scientist Training Program, University of Minnesota, MN, USA.
Summary
Cortical spreading depolarization, a brain phenomenon, also occurs in the retina after vessel occlusion. This retinal spreading depolarization damages tissue, but drugs like MK-801 and sumatriptan can reduce its frequency.
Area of Science:
- Neuroscience
- Ophthalmology
- Pathophysiology
Background:
- Cortical spreading depolarization (CSD) is a known brain event linked to injury.
- Its occurrence and role in the retina remain unexplored in vivo.
- Retinal vascular diseases cause significant vision loss.
Purpose of the Study:
- To investigate if spreading depolarization occurs in the retina in vivo.
- To characterize retinal spreading depolarization properties.
- To explore potential therapeutic interventions targeting retinal spreading depolarization.
Main Methods:
- Induction of retinal vessel occlusion via photothrombosis in rats.
- Monitoring of direct current potential shifts and neuronal activity.
- Measurement of arteriole diameter and tissue oxygen tension.
- Pharmacological modulation using NMDA antagonist MK-801 and sumatriptan.
Main Results:
- Spreading depolarization episodes were successfully generated in the rat retina.
- Retinal spreading depolarization exhibited properties similar to cortical CSD, including propagation speed and physiological changes.
- MK-801 and sumatriptan significantly reduced the frequency of retinal spreading depolarization.
Conclusions:
- Spreading depolarization occurs in the retina in vivo following vascular occlusion.
- Retinal spreading depolarization may contribute to tissue damage in acute retinal ischemia.
- Pharmacological inhibition of retinal spreading depolarization presents a potential therapeutic strategy for vision preservation in conditions like branch retinal vein occlusion.
