Related Experiment Video
Updated: Mar 18, 2026

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
Published on: May 23, 2025
Purification and characterization of glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) from pea seeds
Zahid Gani1, Vishant Mahendra Boradia1, Janaki Raghu Ram1
1National Institute of Pharmaceutical Education and Research, Phase X, Sector 67, SAS Nagar, Punjab, 160067, India.
Abstract:
Glyceraldehyde-3-phosphate dehydrogenase [GAPDH, NAD + oxidoreductase (phosphorylating) 1.2.1.12] catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate coupled with the reduction of NAD(+) to NADH. In addition to its role in glycolysis, this enzyme has numerous alternate functions, in both prokaryotes and eukaryotes. In plants, additional functions have been reported from multiple species including Pisum sativum. A recent study has identified that GAPDH may play an important role in seed ageing and programmed cell death. Despite this the existing purification protocols are almost 40 years old, and only partial characterization of the enzyme has been reported. In the current study, we report a modified method for purification of enzymatically active pea seed GAPDH along with the characterization of the enzyme. Using 2D gel electrophoresis our study also demonstrates that pea seeds contain four isoforms of NAD(+) dependent GAPDH.
Related Concept Videos
Energy-releasing Steps of Glycolysis
The first energy-releasing step—the 6th step of glycolysis...
Glycolysis: Preparatory Phase
Glycolysis: Pay-off Phase
Step 1 - 5: Glycolysis Preparatory Phase
The first phase of glycolysis has 5 steps where the glucose is...
Energy-requiring Steps of Glycolysis
Other Glycolytic Pathways

