Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of the histone core complex.

S Y Chung, W E Hill, P Doty

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Submarine groundwater discharge and associated nutrient influx in surroundings of the estuary region at Gulf of Mannar coast, Indian Ocean.

    Chemosphere·2022
    Same author

    Characteristics of microplastics in the beach sediments of Marina tourist beach, Chennai, India.

    Marine pollution bulletin·2022
    Same author

    Measurement of submarine groundwater discharge (SGD) into Tiruchendur coast at southeast India using <sup>222</sup>Rn as a naturally occurring tracer.

    Marine pollution bulletin·2021
    Same author

    Microplastics and trace metals in fish species of the Gulf of Mannar (Indian Ocean) and evaluation of human health.

    Environmental pollution (Barking, Essex : 1987)·2021
    Same author

    Minimally invasive surgery for massive orbital osteomas.

    European annals of otorhinolaryngology, head and neck diseases·2021
    Same author

    Assessment of groundwater from an industrial coastal area of south India for human health risk from consumption and irrigation suitability.

    Environmental research·2021

    A stable histone core complex, crucial for DNA packaging, exists in equilibrium between tetramer and octamer forms. This finding advances our understanding of chromatin structure and histone organization.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Chromatin Structure

    Background:

    • Histones are fundamental proteins involved in DNA packaging within eukaryotic cells.
    • The histone core complex, composed of histones H2A, H2B, H3, and H4, forms the nucleosome structure.
    • Understanding the quaternary structure of the histone core complex is essential for elucidating chromatin dynamics.

    Purpose of the Study:

    • To isolate and characterize the stable histone core complex from chicken erythrocyte chromatin.
    • To determine the oligomeric state and solution behavior of the histone core complex.
    • To investigate the equilibrium between different histone complex forms.

    Main Methods:

    • Isolation of the histone core complex from chicken erythrocyte chromatin using high salt concentrations.

    Related Experiment Videos

  • Characterization using velocity ultracentrifugation to determine sedimentation properties.
  • Molecular weight determination via low-speed sedimentation equilibrium studies.
  • Analysis of solution behavior using chemical cross-linking experiments.

    • Main Results:

    • A stable histone core complex containing equimolar H2A, H2B, H3, and H4 was successfully isolated.
    • Sedimentation studies revealed a single sharp boundary with a sedimentation coefficient of S020,w = 3.8 +/- 0.1.
    • Sedimentation equilibrium studies indicated an equilibrium between tetrameric (H2A)(H2B)(H3)(H4) and octameric [(H2A)(H2B)(H3)(H4)]2 species.
    • The tetramer molecular weight was determined to be 55,000, with an equilibrium constant K ≈ 1.2 X 10^-5 L/mol at 10°C.
    • Chemical cross-linking experiments corroborated the tetramer-octamer equilibrium in solution.

    Conclusions:

    • The histone core complex exists in a dynamic equilibrium between tetrameric and octameric forms in solution.
    • This equilibrium is a fundamental property of the histone core complex and influences chromatin structure.
    • The findings provide insights into the structural organization and stability of histone complexes.