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Updated: Mar 18, 2026
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using
Ana Lara-Oya1, Javier Moisés Rodríguez-Granger1, María Del Carmen Liébana-Martos1
1Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
Abstract:
We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosissensu stricto, 7 (3.7 %) as Mycobacteriumbovis, 5 (2.6 %) as M. bovis bacillus Calmette-Guérin, 2 (1.1 %) as Mycobacteriumafricanum and 1 (0.5 %) as Mycobacteriumcaprae; no strains belonged to Mycobacteriummicroti and Mycobacteriumcanettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment.
