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The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression

Lei Yu1, Xuejun Gong2, Lei Sun3

  • 1Department of Infectious Disease,The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.

Plos One
|July 9, 2016
PubMed

Insights

Circular RNAs (circRNAs) like Cdr1as are implicated in cancer. This study shows Cdr1as promotes hepatocellular carcinoma (HCC) by downregulating miR-7, offering potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Circular RNAs (circRNAs) regulate gene expression and are implicated in various diseases.
  • Hepatocellular carcinoma (HCC) is a major global health concern with complex molecular underpinnings.
  • The specific role of Cdr1as in HCC pathogenesis requires further investigation.

Purpose of the Study:

  • To elucidate the role of Cdr1as in hepatocellular carcinoma (HCC).
  • To investigate the relationship between Cdr1as, miR-7, and HCC progression.
  • To explore Cdr1as as a potential biomarker and therapeutic target in HCC.

Main Methods:

  • Quantitative real-time PCR to assess Cdr1as and miR-7 expression levels in HCC tissues and adjacent non-tumor tissues.
  • Cell proliferation and invasion assays to evaluate the functional impact of Cdr1as and miR-7.
  • Western blotting to detect the expression of target genes CCNE1 and PIK3CD.

Main Results:

  • Cdr1as expression was significantly upregulated in HCC tissues compared to non-tumor tissues.
  • miR-7 expression was downregulated in HCC tissues and inversely correlated with Cdr1as levels.
  • Knockdown of Cdr1as or overexpression of miR-7 suppressed HCC cell proliferation and invasion, partly via CCNE1 and PIK3CD.
  • Cdr1as knockdown inhibited HCC progression by targeting miR-7.

Conclusions:

  • Cdr1as functions as an oncogene in HCC.
  • Cdr1as promotes HCC cell proliferation and invasion by sponging miR-7.
  • Cdr1as represents a potential diagnostic biomarker and therapeutic target for HCC.

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