Related Experiment Video
Updated: Mar 18, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Novel Functional Genomics Approaches: A Promising Future in the Combat Against Plant Viruses
Vincent N Fondong1, Ugrappa Nagalakshmi1, Savithramma P Dinesh-Kumar1
1First author: Department of Biological Sciences, Delaware State University, Dover; second author: Department of Plant Biology, College of Biological Sciences, University of California, Davis; and third author: Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis.
Abstract:
Advances in functional genomics and genome editing approaches have provided new opportunities and potential to accelerate plant virus control efforts through modification of host and viral genomes in a precise and predictable manner. Here, we discuss application of RNA-based technologies, including artificial micro RNA, transacting small interfering RNA, and Cas9 (clustered regularly interspaced short palindromic repeat-associated protein 9), which are currently being successfully deployed in generating virus-resistant plants. We further discuss the reverse genetics approach, targeting induced local lesions in genomes (TILLING) and its variant, known as EcoTILLING, that are used in the identification of plant virus recessive resistance gene alleles. In addition to describing specific applications of these technologies in plant virus control, this review discusses their advantages and limitations.
More Related Videos
05:56Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Published on: February 27, 2021
08:17VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Published on: August 23, 2013
Related Concept Videos
Subviral Agents
Viral Structure
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...