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Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities
1a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.
Bioscience, Biotechnology, and Biochemistry
|July 10, 2016
Abstract:
Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of α-fucosidase were purified from the viscera of Pomacea canaliculata. The thermostability of F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction and pH stability.
