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Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Published on: August 21, 2019
Influence on radiosensitivity of lung glandular cancer cells when ERCC1 gene silenced by targeted siRNA
1Department of Respiratory Medicine, Zhengzhou Central Hospital, Zhengzhou 450007, China.
Objective:
To identify the influence on radiosensitivity of lung glandular cancer cells when excisions repair cross-complementing group1 (ERCC1) gene was silenced by targeted siRNA.
Methods:
siRNA which targeting to ERCC1 and control siRNA was designed and synthesized. The human lung glandular cancer SPC-A-1 cells was transfected. A total of 56 nude mice were divided into two groups, and two kinds of SPC-A-1 cells were transplanted to armpit of right forelimb, to establish the nude mice subcutaneous xenotransplanted tumor model of human lung glandular cancer cells. After the tumor was developed, the nude mice were randomly divided into four groups and accepted different doses of X-Ray radiation, then the change of tumor volume, survival time of mice in every group were recorded and the average lifetime was calculated. Twenty-one days later of X-ray experiment, two mice were taken and killed in each group and the tumors organizations were stripped. The cell apoptosis rate and cell cycle distributions were obtained by FCM (flow cytometry).
Results:
The volume of tumor which ERCC1 gene was silenced was less than single irradiation group after X-ray irradiation, and the growth speed was slower and the lifetime of mice was lengthened as well (P < 0.05). The cells apoptosis rate and the rate of G2/M cells which ERCC1 gene was silenced were higher than the same dose control group and the rate of G1 cells were lower, which indicated that the cells could be stopped at G2/M point, the cell proliferation was inhibited, the cell apoptosis was promoted and the radiation sensitivity was improved after the ERCC1 was silenced.
Conclusions:
The radiation sensitivity of lung glandular tumor could be improved after the ERCC1 gene was silenced by siRNA.
Insights
Silencing the excision repair cross-complementing group1 (ERCC1) gene with siRNA enhances lung cancer cell radiosensitivity. This approach reduces tumor growth and increases mouse survival time, offering a promising strategy for lung cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Radiotherapy
Background:
- Lung glandular cancer exhibits resistance to radiation therapy.
- The ERCC1 gene plays a role in DNA repair, potentially influencing radiosensitivity.
Purpose of the Study:
- To investigate the impact of silencing the ERCC1 gene on the radiosensitivity of human lung glandular cancer cells.
- To evaluate the therapeutic potential of ERCC1 gene silencing in combination with X-ray irradiation.
Main Methods:
- Human lung glandular cancer SPC-A-1 cells were transfected with ERCC1-targeted siRNA.
- A subcutaneous xenotransplanted tumor model was established in nude mice.
- Mice received varying doses of X-ray radiation, and tumor volume and survival were monitored.
Main Results:
- Silencing ERCC1 significantly reduced tumor volume and growth speed post-irradiation compared to controls.
- Mice treated with ERCC1 silencing and irradiation exhibited longer survival times (P < 0.05).
- ERCC1 silencing increased cancer cell apoptosis and G2/M cell cycle arrest, inhibiting proliferation.
Conclusions:
- Silencing the ERCC1 gene via siRNA can significantly improve the radiosensitivity of lung glandular cancer.
- Targeting ERCC1 presents a potential strategy to enhance the efficacy of radiotherapy for lung cancer.
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