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Updated: Mar 18, 2026
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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
Published on: July 18, 2011
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A Sensitive and Rapid Method for Mapping Protein Bound to DNA by Atomic Force Microscopy
M Tanigawa1, M Machida1,2, T Okada1
1a Joint Research Center for Atom Technology , Higashi 1-1-4, Tsukuba, Ibaraki 305 , Japan.
Bioscience, Biotechnology, and Biochemistry
|July 10, 2016
Abstract:
We addressed the strategies of mapping protein binding sites on a DNA fragment by atomic force microscopy (AFM). The protein binding site was uniquely mapped by distinguishing two termini of a linear DNA fragment. Our simple methods were found very useful to get information on transcription regulatory regions by taking advantage of long range and quick scanning by AFM.
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