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Effects of Substrate Solubility in Interesterification with Triolein by Immobilized Lipase in Supercritical Carbon
S H Yoon1,2, H Nakaya1, O Ito1
1a Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Science, The University of Tokyo.
Abstract:
The interesterification reaction by immobilized lipase between triolein and behenic acid (BA) or ethyl behenate (EB) was investigated in supercritical carbon dioxide (SCCO2) to produce 1,3-dibehenoyl-2-oleoyl glycerol (BOB). The incorporation rate of behenoyl group to triolein was much higher with EB as a substrate than with BA. The solubility in SCCO2 was found to be significantly higher for EB than BA, which seemed to cause the higher formation rate of the behenoyl-enzyme complex in the former case to give the higher production rate of the final interesterification product, BOB.
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