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Inhibition of Glucan Synthesis by Casein Polymers Crosslinked by Glutaraldehyde
O Hayashida1, F Mori1, K Hasumi1
1a Department of Applied Biological Science, Faculty of Agriculture, Tokyo Noko University.
Abstract:
Glutaraldehyde-crosslinked α-casein polymers with molecular masses of 1-5×10(5) were found to inhibit adhesive insoluble glucan formation catalyzed by glucan synthases from Streptococcus sobrinus B13, a cariogenic oral bacterium. Of the three subcomponents of the glucan synthases tested, primer-dependent insoluble glucan synthase was inhibited specifically by the M r~2.7×10(5) polymer at a concentration of 1.4-8.5 μg/ml. The polymer was also active in inhibiting the artificial plaque formation by S. sobrinus B13.
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