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Related Experiment Videos

Late passage cells display an increase in contractile behavior.

J M Gibson1, S B Milam, R J Klebe

  • 1Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.

Mechanisms of Ageing and Development
|May 1, 1989
PubMed
Summary

Aging cells show increased fibronectin and f-actin, enhancing cell migration, spreading, and matrix contraction. These changes in cellular components contribute to functional differences between young and old cells.

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Area of Science:

  • Cell Biology
  • Aging Research
  • Extracellular Matrix Dynamics

Background:

  • Cellular aging is associated with significant changes in the extracellular matrix (ECM) and cytoskeleton.
  • Fibronectin structure and function, as well as f-actin organization, are altered during the aging process.
  • Understanding these alterations is crucial for comprehending age-related cellular dysfunction.

Purpose of the Study:

  • To investigate the functional implications of age-related changes in fibronectin and f-actin.
  • To compare the behavior of young (low population doubling level - PDL) and aged (high PDL) diploid cell strains.
  • To determine how alterations in cellular components affect cell migration, spreading, and ECM interaction.

Main Methods:

  • Utilized diploid cell strains of varying population doubling levels (PDL) to represent young and aged cells.

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  • Assessed cell spreading behavior and migration using a modified phagokinetic track assay.
  • Measured collagen gel contraction capacity and f-actin organization via phalloidin staining.

    • Main Results:

    • High PDL cells exhibited significantly enhanced cell spreading compared to low PDL cells.
    • High PDL cells demonstrated increased migration (phagokinetic activity) and faster collagen gel contraction.
    • Phalloidin staining confirmed substantially greater f-actin content in high PDL cells.

    Conclusions:

    • Aged (high PDL) cells possess greater capacity for force exertion on the ECM.
    • Elevated f-actin content in aged cells likely drives increased cellular contractility.
    • These findings link cytoskeletal changes during aging to enhanced cell spreading, migration, and matrix remodeling.