Evaporation and Hydrocarbon Chain Conformation of Surface Lipid Films

The inhibition of the rate of evaporation (R_evap) by surface lipids is relevant to reservoirs and dry eye. Our aim was to test the idea that lipid surface films inhibit R_evap.

R_evap were determined gravimetrically. Hydrocarbon chain conformation and structure were measured using a Raman microscope. Six 1-hydroxyl hydrocarbons (11-24 carbons in length) and human meibum were studied. Reflex tears were obtained from a 62-year-old male.

The Raman scattering intensity of the lipid film deviated by about 7 % for hydroxyl lipids and varied by 21 % for meibum films across the entire film at a resolution of 5 μm². All of the surface lipids were ordered. R_evap of the shorter chain hydroxyl lipids were slightly (7%) but significantly lower compared with the longer chain hydroxyl lipids. R_evap of both groups was essentially similar to that of buffer. A hydroxyl lipid film did not influence R_evap over an estimated average thickness range of 0.69 to >6.9 μm. R_evap of human tears and buffer with and without human meibum (34.4 μm thick) was not significantly different. R_evap of human tears was not significantly different from buffer.

Human meibum and hydroxyl lipids, regardless of their fluidity, chain length, or thickness did not inhibit R_evap of buffer or tears even though they completely covered the surface. It is unlikely that hydroxyl lipids can be used to inhibit R_evap of reservoirs. Our data do not support the widely accepted (yet unconfirmed) idea that the tear film lipid layer inhibits R_evap of tears.