Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

1.6K
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.6K
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

826
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
826
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

929
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
929
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

855
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
855
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

1.4K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.4K
Respiration Pathways01:26

Respiration Pathways

875
Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
875

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is heptelidic (koningic) acid a microbial hormone that regulates secondary metabolism in the biocontrol fungus Trichoderma virens?

Current genetics·2025
Same author

Trichoderma virens exerts herbicidal effect on Arabidopsis thaliana via modulation of amino acid metabolism.

Plant science : an international journal of experimental plant biology·2023
Same author

Genetic Evidence in Favor of a Polyketide Origin of Acremeremophilanes, the Fungal "Sesquiterpene" Metabolites.

Microbiology spectrum·2022
Same author

Dual role of a dedicated GAPDH in the biosynthesis of volatile and non-volatile metabolites- novel insights into the regulation of secondary metabolism in Trichoderma virens.

Microbiological research·2021
Same author

Structure-function analysis reveals Trichoderma virens Tsp1 to be a novel fungal effector protein modulating plant defence.

International journal of biological macromolecules·2021
Same author

Expression, purification, crystallization and X-ray diffraction studies of a novel root-induced secreted protein from Trichoderma virens.

Acta crystallographica. Section F, Structural biology communications·2020

Related Experiment Video

Updated: Mar 18, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
07:59

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

10.5K

The Terpenoid Biosynthesis Toolkit of Trichoderma.

Ravindra Bansal, Prasun Kumar Mukherjee

    Natural Product Communications
    |July 12, 2016
    PubMed
    Summary

    This study identifies genes for terpene biosynthesis in Trichoderma fungi. While condensation genes are conserved, terpene cyclase numbers vary, impacting secondary metabolite production.

    Area of Science:

    • Mycology
    • Biotechnology
    • Genomics

    Background:

    • The genus Trichoderma comprises fungi vital for biotechnology, known for producing diverse secondary metabolites.
    • Existing genomic data for Trichoderma spp. detail non-ribosomal peptide synthetases and polyketide synthases, but lack comprehensive information on terpenoid biosynthesis pathways.

    Purpose of the Study:

    • To identify and characterize genes involved in terpene biosynthesis across three key Trichoderma species.
    • To compare the genetic machinery for terpenoid production, focusing on terpene cyclases, among T. virens, T. atroviride, and T. reesei.

    Main Methods:

    • Genome-wide analysis of three Trichoderma species (T. virens, T. atroviride, T. reesei).
    • Identification and comparative analysis of genes encoding enzymes for terpene biosynthesis, particularly terpene cyclases.

    More Related Videos

    Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
    08:56

    Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

    Published on: August 16, 2018

    18.3K
    GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
    11:38

    GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

    Published on: October 24, 2011

    16.0K

    Related Experiment Videos

    Last Updated: Mar 18, 2026

    A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
    07:59

    A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

    Published on: October 4, 2019

    10.5K
    Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
    08:56

    Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

    Published on: August 16, 2018

    18.3K
    GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
    11:38

    GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

    Published on: October 24, 2011

    16.0K
  • Investigation of the organization of these genes within secondary metabolism-related gene clusters.

    • Main Results:

    • Genes responsible for the condensation steps of terpene biosynthesis are highly conserved among the studied Trichoderma species.
    • Significant variation was observed in the number and genomic organization of terpene cyclases.
    • T. virens possesses eleven terpene cyclases, T. atroviride has seven, and T. reesei has six, with a subset located in putative secondary metabolism gene clusters.

    Conclusions:

    • The identified terpene biosynthesis genes provide a foundation for understanding terpenoid production in Trichoderma.
    • Variations in terpene cyclase genes suggest species-specific differences in the repertoire and regulation of terpenoid secondary metabolites.
    • This research highlights the genetic diversity of terpenoid biosynthesis pathways within the Trichoderma genus.