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Related Concept Videos

Plaquing of Herpes Simplex Viruses04:41

Plaquing of Herpes Simplex Viruses

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Plaquing is a routine method used to quantify live viruses in a population. Though plaquing is frequently taught in various microbiology curricula with bacteria and bacteriophages, plaquing of mammalian viruses is more complex and time-consuming. This protocol describes the procedures that function reliably for regular work with herpes simplex...
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Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus06:14

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In this manuscript, we describe a simple method of growth, purification, and titration of the oncolytic herpes simplex virus for preclinical use.
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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy06:40

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

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ICP0 undergoes nuclear-to-cytoplasmic translocation during HSV-1 infection. The molecular mechanism of this event is not known. Here we describe the use of confocal microscope as a tool to quantify ICP0 movement in HSV-1 infection, which lays the groundwork for quantitatively analyzing ICP0 translocation in future mechanistic...
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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 111:56

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

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The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of...
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siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells03:44

siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells

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This video demonstrates a small interfering RNA (siRNA)-based technique to interfere with autophagic flux. A siRNA targeting FIP200 mRNA is introduced into monocyte-derived immature dendritic cells (iDCs) using electroporation, causing the knockdown of FIP200 expression and leading to the inhibition of nuclear lamin disruption via autophagy. Upon adding herpes simplex virus (HSV), the nuclear egress of viral progeny is inhibited due to the absence of lamin disruption via autophagic...
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Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells03:43

Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells

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This video describes the virus plaque assay of the infectious herpes simplex virus or HSV. The diluted viral particles are inoculated to the monolayer of epithelial cells. Upon incubation, the virus forms plaques and is counted visually to determine the infectious virus...
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Related Experiment Video

Updated: Jan 20, 2026

Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

6.9K

Autoimmune Post-Herpes Simplex Encephalitis.

Ana B Chelse1, Leon G Epstein1

  • 1Division of Neurology, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL; Departments of Pediatrics and Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL.

Pediatric Neurology Briefs
|July 12, 2016
PubMed
Summary
This summary is machine-generated.

Researchers studied 14 patients with immune-mediating relapsing symptoms of post-herpes simplex encephalitis (HSE). This surveillance identified key characteristics of this rare neurological condition.

Keywords:
AutoimmunityEncephalitisHerpes Simplex Virus

More Related Videos

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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

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Related Experiment Videos

Last Updated: Jan 20, 2026

Plaquing of Herpes Simplex Viruses
04:41

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

6.9K
Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
06:14

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus

Published on: May 13, 2021

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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
11:56

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Published on: August 24, 2015

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Area of Science:

  • Neuroimmunology
  • Infectious Diseases

Background:

  • Post-herpes simplex encephalitis (HSE) can present with immune-mediated relapsing symptoms.
  • A prospective surveillance study was conducted to investigate these specific cases.

Discussion:

  • Understanding the immune mechanisms behind relapsing HSE is crucial for effective treatment.
  • This study highlights the need for continued monitoring of patients with complex neurological presentations.

Key Insights:

  • The study involved 14 patients exhibiting immune-mediating relapsing symptoms of HSE.
  • Data was collected through a prospective surveillance approach.

Outlook:

  • Further research is needed to elucidate the precise immunological pathways involved in relapsing HSE.
  • Developing targeted immunomodulatory therapies could improve outcomes for affected individuals.