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Synthesis, Biological Activity, and Metabolism of 8',8',8'-Trideuteroabscisic Acid
Y Todoroki1, S Nakano1, N Hirai1
1a Division of Applied Life Sciences, Graduate School of Agriculture , Kyoto University , Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto 606-01 , Japan.
Abstract:
An 8',8',8'-trideuterated analog of abscisic acid (ABA) was diastereoselectively synthesized as a new analog of ABA that is resistant to 8'-hydroxyIation, the first metabolic reaction of ABA, owing to the primary kinetic isotope effect. (+)-8',8',8'-Trideutero-ABA showed long-term activity in the rice elongation assay. The rate of metabolism of this analog in rice cell suspension culture was about two fold slower than that of (+)-ABA. The concentration of 8',8'-dideuterophaseic acid produced was about 1/3 that of phaseic acid converted from (+)-ABA. This result indicated that the long-lasting activity of the (+)-trideutero-ABA in the rice assay was the result of the delayed 8'-hydroxylation as expected.
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