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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
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Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates
Marie Cellier1, Arthur L James2, Jonathan Lowe2
1Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
Bioorganic & Medicinal Chemistry
|July 12, 2016
Abstract:
A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.

