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A PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation.

Huei-Wen Wu1, Yi-Hsing Hsiao2, Chih-Chen Chen3,4

  • 1Institutes of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Zhunan 35053, Taiwan. fecancer@gmail.com.

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|July 12, 2016
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Summary
This summary is machine-generated.

We developed a microfluidic chip for high-throughput embryoid body (EB) formation, overcoming limitations of the traditional hanging drop method. This novel approach enables efficient medium exchange and on-chip characterization of EBs.

Keywords:
embryoid bodyembryonic stem cellmicrofluidic hanging drop

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Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Microfluidics

Background:

  • The hanging drop technique is standard for embryoid body (EB) formation but is labor-intensive and hinders medium exchange.
  • Limitations in current methods impede high-throughput culturing and characterization of EBs.

Purpose of the Study:

  • To develop a microfluidic chip for efficient, high-throughput formation of embryoid bodies (EBs).
  • To enable on-chip medium exchange and subsequent characterization of EBs.

Main Methods:

  • Fabrication of a microfluidic chip using polydimethyl-siloxane (PDMS) replicated from an SU-8 mold on a glass substrate.
  • Optimization of droplet formation using varying hydrostatic pressures in microfluidic channels with 1000 μm diameter openings.
  • Culturing of mouse embryonic stem cells within droplets for EB development.

Main Results:

  • Successful high-throughput formation of EBs using the microfluidic chip.
  • Demonstration of efficient medium exchange capabilities within the chip.
  • Feasibility of on-chip immunochemistry staining and EB characterization.

Conclusions:

  • The microfluidic chip offers a significant advancement over traditional methods for EB formation.
  • This technology facilitates high-throughput culturing, medium exchange, and on-chip analysis of EBs.
  • The developed system streamlines stem cell culture and characterization processes.