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Recombinant Ranaviruses for Studying Evolution of Host-Pathogen Interactions in Ectothermic Vertebrates
Jacques Robert1, James K Jancovich2
1Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA. Jacques_Robert@urmc.rochester.edu.
Viruses
|July 12, 2016
Summary
Ranaviruses cause emerging infectious diseases in cold-blooded vertebrates. This review details methods for creating ranavirus recombinants to study their biology in vitro and in vivo.
Area of Science:
- Virology
- Infectious Diseases
- Molecular Biology
Background:
- Ranaviruses (family Iridoviridae) are large DNA viruses responsible for emerging infectious diseases in cold-blooded vertebrates globally.
- While some aspects of ranavirus biology are known, their replication, host interactions, and evolution, particularly in vivo, remain poorly understood.
Purpose of the Study:
- To review strategies for generating site-specific ranavirus recombinants.
- To discuss the characterization and utility of these recombinants in research.
Main Methods:
- Review of established and novel techniques for creating recombinant ranaviruses.
- Analysis of studies utilizing ranavirus recombinants for in vitro and in vivo investigations.
Main Results:
- Development of methods for generating ranavirus recombinants, including those with reporter genes or gene deletions.
- Demonstration of the utility of these recombinants in elucidating viral mechanisms and pathogenesis.
Conclusions:
- Site-specific recombinant technology is crucial for advancing the study of ranaviruses.
- These tools facilitate a deeper understanding of ranavirus replication, host interactions, and evolution, aiding in disease management.
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