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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

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Related Experiment Video

Updated: Mar 18, 2026

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Published on: December 16, 2013

Tumor-induced stromal reprogramming drives lymph node transformation.

Angela Riedel¹, David Shorthouse¹, Lisa Haas¹

¹MRC Cancer Unit, University of Cambridge, Cambridge, UK.

Nature Immunology

|July 12, 2016

Summary

This summary is machine-generated.

Tumor-draining lymph node (TDLN) fibroblastic reticular cells (FRCs) proliferate and remodel their structure in response to cancer. This adaptation alters immune cell function, potentially promoting tumor immunosuppression.

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Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

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Last Updated: Mar 18, 2026

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

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Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Area of Science:

Immunology
Cancer Biology
Cell Biology

Background:

Lymph node (LN) stromal cells, especially fibroblastic reticular cells (FRCs), are crucial for immune regulation and LN structure.
Tumor metastasis to LNs often predicts a poor prognosis.
The role of stromal cells in the tumor-draining lymph node (TDLN) microenvironment is not well understood.

Purpose of the Study:

To investigate the contribution of FRCs to the TDLN microenvironment during tumor progression.
To understand how tumor-derived factors influence FRC behavior and function within TDLNs.

Main Methods:

Comparative transcriptional analysis of FRCs from TDLNs and non-draining LNs.
Analysis of FRC proliferation and network remodeling in response to tumor cues.

Main Results:

FRCs in TDLNs proliferated and their network structure was remodeled upon exposure to tumor-derived factors.
Transcriptional analysis revealed reprogramming of pathways related to matrix remodeling, chemokine/cytokine signaling, and leukocyte interactions.
Downregulation of FRC-derived CCL21 and IL-7 was observed, leading to altered immune cell composition and localization.

Conclusions:

TDLN FRCs adapt to tumor presence by altering their structure and function.
These adaptations in the LN stroma contribute to an immunosuppressive microenvironment.
Understanding these stromal changes is critical for developing strategies to combat cancer metastasis.