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Updated: Mar 18, 2026
Potato Virus X-Based microRNA Silencing VbMS In Potato.
Published on: May 11, 2020
Development of reverse transcription loop-mediated isothermal amplification assay as a simple detection method of
Ryoji Suzuki1, Shiro Fukuta1, Yuho Matsumoto1
1Aichi Agricultural Research Center, 1-1 Sagamine, Nagakute, Aichi 480-1193, Japan.
Abstract:
For a simple and rapid detection of Chrysanthemum stem necrosis virus (CSNV) from chrysanthemum and tomato, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed. A primer set designed to the genome sequences of CSNV worked most efficiently at 63°C and could detect CSNV RNA within 12min by fluorescence monitoring using an isothermal DNA amplification and fluorescence detection device. The result of a specificity test using seven other viruses and one viroid-infectable chrysanthemum or tomato showed that the assay could amplify CSNV specifically, and a sensitivity comparison showed that the RT-LAMP assay was as sensitive as the reverse transcriptase polymerase chain reaction. The RT-LAMP assay using crude RNA, extracted simply, could detect CSNV. Overall, the RT-LAMP assay was found to be a simple, specific, convenient, and time-saving method for CSNV detection.
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