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Related Experiment Video

Updated: Mar 18, 2026

A View of Their Own: Capturing the Egocentric View of Infants and Toddlers with Head-Mounted Cameras

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Published on: October 5, 2018

Early Perspective.

Brinton Seashore-Ludlow^1,2, Thomas Lundbäck^1,2

¹1 Chemical Biology Consortium Sweden, Science for Life Laboratories, Stockholm, Sweden.

Journal of Biomolecular Screening

|July 13, 2016

Summary

The cellular thermal shift assay (CETSA) is a method to detect drug binding by measuring protein stability. Microplate formatting enhances its use in drug discovery and chemical probe validation.

Keywords:

CETSA chemical probe validation protein unfolding structure–activity relationship target engagement

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Area of Science:

Biophysics
Pharmacology
Biochemistry

Background:

The cellular thermal shift assay (CETSA) measures drug-target interactions by detecting ligand-induced protein stabilization.
CETSA is applicable to various biological matrices, including cell lysates, live cells, and tissues.
Intracellular soluble proteins are the primary targets for CETSA applications.

Purpose of the Study:

To review advancements in adapting CETSA to microplate formats.
To discuss the benefits and challenges of microplate-formatted CETSA.
To forecast future applications of microplate-formatted CETSA in drug discovery.

Main Methods:

Adaptation of the original CETSA procedure from PCR tubes and Western blot detection.
Development of a homogeneous assay in high-density microplates.
Implementation of high-throughput formats for systematic drug discovery applications.

Main Results:

Microplate formatting enables homogeneous CETSA detection.
High-throughput formats facilitate systematic studies of structure-activity relationships.
Knowledge gained from microplate formatting is crucial for broader CETSA application.

Conclusions:

Microplate formatting is a key technological development for CETSA.
High-throughput CETSA is essential for drug discovery and chemical probe validation.
Further research will expand the utility and applications of CETSA.