Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vascular endothelial integration of multiple biophysical stimuli.

Frontiers in cardiovascular medicine·2026
Same author

A computational model of chemically- and mechanically-induced thrombus formation in cerebral aneurysms.

Computers in biology and medicine·2026
Same author

In situ impedance analysis device for clot characterization in large vessel occlusion acute ischemic stroke, a first-in-human study.

Journal of neurointerventional surgery·2026
Same author

A case report of nonrheumatic myocarditis following group G streptococcus pharyngitis.

Radiology case reports·2026
Same author

Modeling the effect of substrate topography on cellular and nuclear deformations.

Computers in biology and medicine·2026
Same author

Noninvasive real-time monitoring of cellular spatiotemporal dynamics via machine learning-enhanced electrical impedance spectroscopy.

Science advances·2025

Related Experiment Video

Updated: Mar 18, 2026

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
11:08

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

16.7K

A simple microfluidic device to study cell-scale endothelial mechanotransduction.

Julie Lafaurie-Janvore1, Elizabeth E Antoine1, Sidney J Perkins1,2

  • 1Laboratoire d'Hydrodynamique de l'École polytechnique, CNRS-EP UMR 7646, Palaiseau, France.

Biomedical Microdevices
|July 13, 2016
PubMed
Summary

This study introduces a microfluidic device to investigate how endothelial cell (EC) shape influences their response to blood flow, revealing cell polarization dictates calcium wave direction.

Keywords:
AtherosclerosisCalcium signalingMechanobiologyMicrofluidic flow chamberMicropatternsShear stress

More Related Videos

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

4.0K
Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
10:35

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

9.4K

Related Experiment Videos

Last Updated: Mar 18, 2026

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
11:08

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

16.7K
A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

4.0K
Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli
10:35

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

9.4K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cardiovascular Research

Background:

  • Atherosclerosis pathogenesis involves chronic inflammation of arterial endothelial cells (ECs).
  • EC shape and mechanotransduction are critical in disturbed blood flow regions where atherosclerosis develops.

Purpose of the Study:

  • To develop a microfluidic device for studying the relationship between endothelial cell shape and mechanotransduction.
  • To investigate how fluid shear stress affects endothelial cell behavior and signaling.

Main Methods:

  • Utilized adhesive micropatterns to control endothelial cell shape and orientation.
  • Integrated a microfluidic chamber for precise flow control and live-cell imaging.
  • Employed micro particle image velocimetry and in situ immunostaining for analysis.

Main Results:

  • Endothelial cells locally amplified shear stress compared to acellular regions.
  • Observed flow-induced lamellipodia formation and cell retraction.
  • Quantified flow-induced calcium mobilization and demonstrated cell polarization-dependent calcium wave propagation.

Conclusions:

  • The developed microfluidic device effectively studies endothelial cell shape and mechanosensitivity.
  • Cellular response to shear stress is significantly influenced by cell morphology and polarization.
  • Intracellular calcium wave propagation is governed by cell polarization, not solely by flow direction.