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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Mass spectrometric epitope mapping.

Kwabena F M Opuni¹, Mahmoud Al-Majdoub¹, Yelena Yefremova¹

¹Proteome Center Rostock, University Medicine and Natural Science Faculty, University of Rostock, Rostock, Germany.

Mass Spectrometry Reviews

|July 13, 2016

Summary

Mass spectrometric epitope mapping precisely identifies antibody-binding sites on antigens. This mature technique offers rapid, sensitive analysis, paving the way for clinical applications in patient stratification.

Keywords:

assembled epitope conformational epitope consecutive epitope discontinuous epitope epitope excision epitope extraction linear epitope mass spectrometry

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Area of Science:

Biochemistry
Immunology
Analytical Chemistry

Background:

Mass spectrometric epitope mapping precisely determines antigen structures interacting with antibodies in solution.
Methods have evolved since the 1990s, enabling analysis of larger antigens and shorter epitope peptides (10-15 amino acids).
Both monoclonal and polyclonal antibodies are applicable, with antibody resistance to proteolysis crucial for key methods.

Purpose of the Study:

To review the advancements and current state of mass spectrometric epitope mapping.
To highlight the method's versatility, sensitivity, and speed.
To anticipate future clinical applications.

Main Methods:

Epitope excision and epitope extraction are primary methods, relying on antibody resistance to enzymatic proteolysis.
In-solution sample handling is critical for successful mapping.
Mass spectrometry serves as the most suitable and rapid read-out technique.

Main Results:

The technique has matured, becoming accessible for any mass spectrometry laboratory.
Sample consumption is remarkably low, down to picomole amounts for both antigen and antibody.
The speed of mass spectrometric epitope mapping surpasses alternative methods.

Conclusions:

Mass spectrometric epitope mapping is a highly advanced and versatile technique.
Its speed, sensitivity, and low sample requirements make it superior to other methods.
Future applications in clinical settings, such as patient characterization and stratification, are expected.