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Reference Values of Reticulocyte Hemoglobin Content and Their Relation With Other Indicators of Iron Status in

Encarnación López-Ruzafa1, Maria A Vázquez-López, Francisco Lendinez-Molinos

  • 1Department of Pediatrics, Torrecárdenas Hospital, Almería, Spain.

Journal of Pediatric Hematology/Oncology
|July 13, 2016
PubMed

Insights

Reticulocyte hemoglobin content (CHr) reference intervals were established for children aged 1-11 years. Age significantly influences CHr, necessitating age-specific reference ranges for accurate assessment of iron status.

Area of Science:

  • Pediatric Hematology
  • Iron Metabolism
  • Clinical Chemistry

Background:

  • Reticulocyte hemoglobin content (CHr) is a key indicator of functional iron deficiency.
  • CHr is understudied in pediatric populations, limiting its clinical utility in children.
  • Establishing reference intervals is crucial for accurate diagnosis and management.

Purpose of the Study:

  • Determine reference intervals for CHr in healthy children aged 1 to 11 years.
  • Investigate the relationship between CHr, iron parameters, and erythropoiesis.
  • Identify factors influencing CHr variability in children.

Main Methods:

  • Cross-sectional study of 902 healthy children (1-11 years).
  • Comprehensive analysis including complete blood count, iron status markers (ferritin, transferrin saturation), erythrocyte protoporphyrin, erythropoietin, C-reactive protein, and CHr.
  • Linear multiple regression analysis to identify CHr determinants.

Main Results:

  • Mean CHr was 30.9±1.8 pg (reference range: 26.9-34.3 pg).
  • CHr values increased with age, necessitating age-specific reference ranges.
  • Age, nutritional status, hematologic measurements, reticulocytes, transferrin saturation, and erythrocyte protoporphyrin explained 39% of CHr variability.

Conclusions:

  • Established age-specific reference intervals for CHr in children.
  • Highlights the significant influence of age and other factors on CHr.
  • Provides a foundation for utilizing CHr in pediatric iron status assessment.

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