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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
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CAT7 and cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function during Human and Zebrafish

Mridula K Ray1, Ole Wiskow2, Matthew J King3

  • 1From the Department of Molecular Biology, Massachusetts General Hospital, and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114.

The Journal of Biological Chemistry
|July 14, 2016
PubMed
Summary

Polycomb Repressive Complex 1 (PRC1) interacts with long non-coding RNAs (lncRNAs) to regulate gene expression. The novel lncRNA CAT7 guides PRC1 activity at specific gene loci during development.

Keywords:
CsCl density gradientPRC1chromatinchromatin regulationdevelopmentgene expressionlong noncoding RNA (long ncRNA, lncRNA)mammalpolycombzebrafish

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Area of Science:

  • Epigenetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Polycomb Repressive Complex 1 (PRC1) is crucial for development and gene silencing.
  • The role of long non-coding RNAs (lncRNAs) in guiding PRC1 activity is largely unknown.
  • Identifying specific lncRNAs that interact with PRC1 is essential for understanding its regulatory mechanisms.

Purpose of the Study:

  • To screen for and identify lncRNAs that co-precipitate with PRC1 from chromatin.
  • To investigate the function of novel lncRNAs in regulating polycomb group protein (PcG)-mediated gene expression.
  • To elucidate the interaction between lncRNAs and PRC1 in the context of gene regulation during neuronal differentiation.

Main Methods:

  • Chromatin co-immunoprecipitation screening for lncRNA-PRC1 interactions.
  • In vivo functional assays to assess the impact of lncRNAs on gene expression.
  • Zebrafish (Danio rerio) embryogenesis assays to study conserved lncRNA function and genetic interactions with PRC1 components.

Main Results:

  • Identified novel lncRNA candidates that co-precipitate with PRC1 and affect PcG-regulated gene expression.
  • Discovered CAT7, a lncRNA regulating the MNX1 locus during neuronal differentiation by influencing PRC1 binding.
  • Demonstrated that zebrafish cat7l genetically interacts with PRC1, with conserved function to human CAT7.

Conclusions:

  • PRC1 functions in concert with specific lncRNAs to achieve precise gene repression.
  • CAT7 and cat7l represent convergent lncRNAs that have independently evolved to fine-tune PRC1 repression at distinct loci.
  • This study provides a framework for understanding lncRNA-mediated epigenetic regulation by PRC1.