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Related Experiment Videos

Antigen detection in onchocerciasis: correlation with worm burden.

M A Schlie-Guzmán1, A R Rivas-Alcalá

  • 1Centro de Investigaciones Ecologicas del Sureste, Carretera Panamericana y Periférico Sur S/N, Chiapas, Mexico.

Tropical Medicine and Parasitology : Official Organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft Fur Technische Zusammenarbeit (GTZ)
|March 1, 1989
PubMed
Summary

An indirect ELISA test effectively detects Onchocerca volvulus antigen in serum and urine. This diagnostic tool aids in onchocerciasis surveys and treatment campaigns in endemic areas.

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Area of Science:

  • Medical Parasitology
  • Immunodiagnostics
  • Public Health

Background:

  • Onchocerciasis, caused by Onchocerca volvulus, is a significant neglected tropical disease.
  • Accurate diagnostic methods are crucial for controlling onchocerciasis in endemic regions like Chiapas, Mexico.

Purpose of the Study:

  • To evaluate the efficacy of an indirect ELISA test for detecting O. volvulus antigen.
  • To assess the utility of serum and urine samples for ELISA-based onchocerciasis diagnosis.
  • To correlate ELISA results with clinical signs and parasitological findings.

Main Methods:

  • Indirect ELISA test performed on serum and urine samples from 169 individuals in an endemic focus.
  • Physical examinations for onchocerciasis clinical signs (nodules, skin lesions, ocular damage).

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  • Microfilariae detection in skin biopsies.

    • Main Results:

    • High sensitivity of the ELISA test: 92.3% for serum and 85.9% for urine.
    • Good correlation found between ELISA positivity and the number of skin microfilariae.
    • Strong correlation (r=0.76) between ELISA test values in serum and urine.

    Conclusions:

    • The indirect ELISA test is a sensitive and reliable method for O. volvulus antigen detection.
    • Serum and urine ELISA provide valuable data for seroepidemiological surveys.
    • This diagnostic approach can guide onchocerciasis mass treatment campaign strategies.