Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

963
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
963
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Examining Epilepsy in Angelman Syndrome: Insights From Caregiver-Reported Data in the Linking Angelman and Dup15q Data for Expanded Research Database.

Pediatric neurology·2026
Same author

Long-term safety and efficacy of deoxycytidine/deoxythymidine in treatment of POLG-related disorders.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Education Research: Defining Competencies and Consensus in Canadian Epilepsy Fellowship Training: A Thematic Discourse Analysis of Expert Perspectives.

Neurology. Education·2026
Same author

Management of ring chromosome 20 syndrome: Narrative review and consensus recommendations.

Epilepsia·2026
Same author

Preparation and Assembly of the Axial Invasion Chamber for Live-Cell Invadopodia Imaging.

Bio-protocol·2026
Same author

Self-limited neonatal epilepsy with 2q24.3 duplications: Case series and literature review.

Epileptic disorders : international epilepsy journal with videotape·2026
Same journal

The Outcome of Cardiac Hydatid Surgery in The Iraqi Center of Heart Diseases.

F1000Research·2026
Same journal

Perception of body donation among the Phase-1 medical students, a questionnaire-based study.

F1000Research·2026
Same journal

Exploring Infertility in Saudi Arabia: Qualitative Insights into IVF Treatment Services and Policy Recommendations.

F1000Research·2026
Same journal

Cyber Military Operations under International Humanitarian Law: Interpreting the Concept of "Attack" and Challenges in Protecting Civilians.

F1000Research·2026
Same journal

Sentiment Analysis of Acceptance TVET Online Courses on the Skill Academy App from Google Play: Leveraging Text Mining with Comparison Machine Learning Model.

F1000Research·2026
Same journal

Emotional intelligence: An important skill to learn now more than ever.

F1000Research·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

1.9K

Recent advances in imaging subcellular processes.

Kenneth A Myers1, Christopher Janetopoulos1

  • 1Department of Biological Sciences, University of the Sciences in Philadelphia, Philadelphia, PA, USA.

F1000Research
|July 14, 2016
PubMed
Summary
This summary is machine-generated.

Advanced microscopy enables cell biology discoveries by visualizing subcellular structures and dynamics. New imaging systems drive research into the spatial and temporal mechanisms controlling molecular cell biology.

Keywords:
STEDStimulated emission-depletion microscopycell biologyimaging subcellular processesmicroscopemicroscopy techniques

More Related Videos

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

2.4K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

12.5K

Related Experiment Videos

Last Updated: Mar 18, 2026

Subcellular Imaging of Neuronal Calcium Handling In Vivo
07:14

Subcellular Imaging of Neuronal Calcium Handling In Vivo

Published on: March 17, 2023

1.9K
Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

2.4K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

12.5K

Area of Science:

  • Cell Biology
  • Microscopy
  • Biotechnology

Background:

  • Cell biology's foundation lies in the ability to visualize cells and their internal structures.
  • Early microscopy allowed observation of organelles, leading to initial understanding of cell function.
  • Dynamic visualization of subcellular architecture fuels current research questions.

Purpose of the Study:

  • To highlight the impact of advanced microscopy on cell biology.
  • To discuss the role of visualization in understanding cellular mechanisms.
  • To emphasize the ongoing development of imaging systems for molecular cell biology.

Main Methods:

  • Advancements in microscopy techniques.
  • Development of fluorescent labeling.
  • Integration of sensitive and fast cameras.
  • Innovation in optical imaging techniques.

Main Results:

  • Enabled detailed observation of subcellular structures and cellular functions.
  • Facilitated a deeper understanding of the dynamic architecture within cells.
  • Led to an explosion of new light microscopic techniques.

Conclusions:

  • Visualization is key to understanding cell biology.
  • Technological advancements in imaging are crucial for molecular cell biology research.
  • Ongoing development of powerful imaging systems will further unravel cellular mechanisms.