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Related Experiment Video

Updated: Mar 18, 2026

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Transcriptomic analyses of Hand2 transgenic embryos.

Noriko Funato1, Hiroki Kokubo2, Yumiko Saga3

  • 1Department of Signal Gene Regulation, Research Center for Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

Genomics Data
|July 14, 2016
PubMed
Summary
This summary is machine-generated.

The Hand2 transcription factor is crucial for jaw development. Altered Hand2 expression in neural crest cells causes upper jaw to transform into lower jaw, revealing key downstream genes.

Keywords:
CAG, the CMV early enhancer/chicken β actinCAT, chloramphenicol acetyl transferaseCraniofacial developmentEvolutionJawMandibleNeural crestVertebratesaRNA, amplified RNAbHLH, basic helix-loop-helix

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Area of Science:

  • Developmental biology
  • Genetics
  • Molecular biology

Background:

  • The transcription factor Hand2 plays a role in embryonic development.
  • Understanding Hand2's downstream targets is essential for elucidating jaw formation mechanisms.

Purpose of the Study:

  • To provide data on gene expression changes related to Hand2 function in jaw development.
  • To identify downstream genes regulated by Hand2 in neural crest cells.

Main Methods:

  • Generated double-transgenic mice (Hand2 (NC)) by crossing specific mouse lines for conditional Hand2 activation.
  • Analyzed gene expression using Affymetrix microarrays.
  • Compared gene expression profiles between wild-type and Hand2 (NC) mutant embryos.

Main Results:

  • Conditional activation of Hand2 in neural crest cells led to upper jaw to lower jaw transformation in mutant mice.
  • Identified differentially expressed genes between wild-type and Hand2 (NC) mutant embryos.
  • Raw transcriptome data is publicly available on NCBI GEO (GSE75805).

Conclusions:

  • Hand2 is a key regulator specifying jaw identity.
  • The provided data offers insights into Hand2-mediated gene regulation during craniofacial development.