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DNA damage response and repair data with pharmacological modulators of Tousled

Prakash Srinivasan Timiri Shanmugam1, Renjith P Nair1, Arrigo DeBenedetti1

  • 1Department of Biochemistry and Molecular Biology, USA.

Data in Brief
|July 14, 2016
PubMed

Insights

Human Tousled kinase 1 (TLK1) is vital for DNA repair and cell survival after radiation. Its activity modulation impacts DNA break repair and recovery in salivary cells.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Radiation Biology

Background:

  • Human Tousled kinase 1 (TLK1) is crucial for DNA repair, replication, and chromatin remodeling.
  • TLK1 activity is transiently downregulated following genotoxic stress, with recovery essential for cell survival.
  • Small molecule modulators of TLK1 offer a tool to investigate its role in DNA damage response.

Purpose of the Study:

  • To elucidate the role of TLK1 in DNA damage response and repair.
  • To investigate the impact of TLK1 activators and inhibitors on human salivary acinar cell survival.
  • To complement existing research on TLK1 activators in salivary gland cells.

Main Methods:

  • Utilized gallic acid (GA) as a TLK1 activator and thioridazine (THD) as an inhibitor.
  • Assessed the impact of GA and THD on TLK1 activity in human salivary acinar cells.
  • Evaluated the role of TLK1 in DNA break repair and cell survival post-radiation.

Main Results:

  • TLK1 plays a significant role in DNA break repair.
  • Modulation of TLK1 activity affects the survival of human salivary acinar cells.
  • Gallic acid and thioridazine differentially impact TLK1-mediated DNA repair pathways.

Conclusions:

  • TLK1 is a key regulator of DNA repair and cell survival following genotoxic insult.
  • Pharmacological targeting of TLK1 can influence DNA repair efficiency and cellular response to radiation.
  • Understanding TLK1's role is critical for developing therapeutic strategies against radiation-induced damage.
Keywords:
DNA repairGallic acidIonizing radiationThioridazineTousled

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