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MiR-27a-3p functions as an oncogene in gastric cancer by targeting BTG2

Lin Zhou1, Xin Liang2, Lingling Zhang2

  • 1Department of Laboratory Medicine, Changzhen Hospital, Second Military Medical University, Shanghai 200003, China.

Oncotarget
|July 14, 2016
PubMed

Insights

MicroRNA-27a-3p (miR-27a-3p) promotes gastric cancer by targeting B-cell translocation gene 2 (BTG2). This miR-27a-3p/BTG2 axis is a potential diagnostic biomarker and therapeutic target for gastric cancer.

Area of Science:

  • Molecular biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-27a (miR-27a) is dysregulated in human cancers, including gastric cancer.
  • B-cell translocation gene 2 (BTG2) plays a role in gastric carcinogenesis.
  • The relationship between miR-27a and BTG2 in gastric cancer remains unexplored.

Purpose of the Study:

  • To investigate the role of miR-27a isoforms in gastric cancer.
  • To identify the target gene of miR-27a in gastric cancer.
  • To elucidate the functional mechanism of the miR-27a/BTG2 axis in gastric carcinogenesis.

Main Methods:

  • Quantitative real-time PCR to measure miR-27a and BTG2 expression.
  • In vitro cell proliferation assays and in vivo tumor growth studies.
  • Western blotting and luciferase reporter assays to confirm target interaction and pathway analysis.

Main Results:

  • Both miR-27a-5p and miR-27a-3p were overexpressed in gastric cancer tissues and cell lines, with miR-27a-3p showing higher expression.
  • Overexpression of miR-27a-3p promoted gastric cancer cell proliferation and tumor growth.
  • BTG2 was identified as a direct target of miR-27a-3p; miR-27a-3p inhibition upregulated BTG2, which induced cell cycle arrest, apoptosis, and inhibited C-myc activation via the Ras/MEK/ERK pathway.

Conclusions:

  • The miR-27a-3p/BTG2 axis plays a critical role in gastric carcinogenesis and progression.
  • miR-27a-3p promotes gastric cancer by downregulating BTG2.
  • The miR-27a-3p/BTG2 axis represents a potential diagnostic biomarker and therapeutic target for gastric cancer.
Keywords:
BTG2apoptosiscell proliferationgastric cancermiR-27a-3p

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