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Spotlight on the three main hepatic fibrogenic cells in HCV-infected patients: Multiple immunofluorescence and

Soheir S Mansy1, Mona M Nosseir2, May M Othman1

  • 1a Electron Microscopy Research Department (Pathology) , Theodor Bilharz Research Institute , Giza , Egypt.

Ultrastructural Pathology
|July 14, 2016
PubMed

Insights

This study reveals myofibroblasts (MF) in liver portal vessels of hepatitis C virus (HCV) patients, prompting a reevaluation of bone marrow fibrocytes and MF behavior in hepatic fibrosis.

Area of Science:

  • Hepatology
  • Cell Biology
  • Immunology

Background:

  • Hepatic fibrosis progression in Hepatitis C Virus (HCV) patients is complex.
  • The precise in vivo interrelationships of hepatic stellate cells, myofibroblasts (MF), and fibroblasts remain inadequately understood.
  • Understanding these fibrogenic cells is crucial for managing liver disease.

Purpose of the Study:

  • To simultaneously investigate the ultrastructure and triple immunofluorescence of hepatic stellate cells, myofibroblasts (MF), and fibroblasts.
  • To elucidate the in vivo interrelation and behavior of these cells during hepatic fibrosis progression in HCV patients.
  • To identify and characterize MF in liver portal vessels.

Main Methods:

  • Simultaneous ultrastructure and triple immunofluorescence studies.
  • Analysis of liver biopsies from HCV RNA positive patients.
  • Morphological characterization of fibrogenic cells, including MF and bone marrow fibrocytes.

Main Results:

  • For the first time, cells with MF morphological characteristics, distinct from bone marrow fibrocytes, were identified in liver portal vessels.
  • The distribution and cellular interrelations of MF were highlighted.
  • The fate of MF in the context of hepatic fibrosis was investigated.

Conclusions:

  • The presence of MF in liver portal vessels necessitates a reevaluation of current knowledge regarding bone marrow fibrocytes.
  • This study provides novel insights into the behavior and interrelationships of key fibrogenic cells in HCV-induced liver fibrosis.
  • Further research is warranted to fully understand the role of MF in fibrogenesis.

Keywords:
Bone marrow fibrocytefibroblasthepatic stellate cellliver fibrosismyofibroblast

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