Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
X-ray Crystallography02:18

X-ray Crystallography

26.6K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.6K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.1K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

C-reactive Protein-to-Albumin Ratio for Evaluating Patients with Chronic Limb-Threatening Ischemia Following Endovascular Therapy.

Journal of atherosclerosis and thrombosis·2026
Same author

Three-dimensional imaging of sulfur chemical states in polymers with micrometer thickness using sulfur K-edge ptychographic tomography.

Scientific reports·2026
Same author

CT-guided PCI: From diagnosis to treatment planning.

Journal of cardiology·2026
Same author

Integrated Echo-Hemodynamic Framework for VA-ECMO in Right Ventricular Infarction.

JACC. Case reports·2026
Same author

Usefulness of the Geriatric Nutritional Risk Index for Assessing Outcomes in Lower Extremity Artery Disease Following Endovascular Therapy.

Circulation reports·2026
Same author

Multidimensional Tracking and Clustering of Electrochemical Reactions in the Positive Electrode of Lithium-Ion Batteries via Operando X‑ray Nanoscale Chemical Imaging.

Chemical & biomedical imaging·2026

Related Experiment Video

Updated: Mar 18, 2026

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
08:44

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

8.2K

Multiple defocused coherent diffraction imaging: method for simultaneously reconstructing objects and probe using

Makoto Hirose, Kei Shimomura, Akihiro Suzuki

    Optics Express
    |July 14, 2016
    PubMed
    Summary

    Multiple defocused coherent X-ray diffraction imaging (CXDI) overcomes sample size limitations with X-ray free electron lasers (XFELs). This novel method reconstructs objects and probes without prior knowledge, enabling broader applications.

    More Related Videos

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
    09:49

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

    Published on: October 23, 2018

    16.6K
    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
    10:12

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

    Published on: June 19, 2018

    9.7K

    Related Experiment Videos

    Last Updated: Mar 18, 2026

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene
    08:44

    Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

    Published on: August 22, 2017

    8.2K
    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
    09:49

    An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

    Published on: October 23, 2018

    16.6K
    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples
    10:12

    Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples

    Published on: June 19, 2018

    9.7K

    Area of Science:

    • Coherent X-ray imaging
    • Diffraction physics
    • X-ray microscopy

    Background:

    • Single-shot coherent X-ray diffraction imaging (CXDI) is limited by sample size relative to the X-ray beam focal spot.
    • This limitation restricts the application of X-ray free electron lasers (XFELs) for imaging larger structures.

    Purpose of the Study:

    • To develop a novel CXDI method that overcomes the sample size limitation.
    • To enable simultaneous reconstruction of objects and the X-ray probe without prior information.

    Main Methods:

    • Proposed multiple defocused CXDI, illuminating isolated objects with a larger divergent beam.
    • Recorded coherent diffraction patterns sequentially for each object.
    • Performed computer simulations and a proof-of-principle experiment at SPring-8.

    Main Results:

    • Successfully demonstrated the multiple defocused CXDI method.
    • Achieved simultaneous reconstruction of objects and the X-ray probe.
    • Validated the ability to image broader samples than previously possible.

    Conclusions:

    • Multiple defocused CXDI effectively overcomes the sample size limitation in XFEL-based imaging.
    • The method allows for simultaneous object and probe characterization.
    • This technique broadens the applicability of CXDI for diverse sample types and XFEL beam analysis.