Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BrainCL: Transformer-Based Brain Network Contrastive Learning with Multi-Order Topology and Salience Masking.

IEEE transactions on medical imaging·2026
Same author

[Enhancing heterologous expression of bovine casein macropeptide <i>via</i> promoter engineering].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2026
Same author

Effects of compound probiotics on intestinal and liver injury in Lohmann Pink chickens challenged by lipopolysaccharide.

Poultry science·2026
Same author

Hepatic Steatosis Severity Prediction in Nonobese Individuals: Machine Learning Model Development and Validation.

Journal of medical Internet research·2026
Same author

A viral protein-driven ubiquitination switch fine-tunes chloroplast outer envelope protein homeostasis.

The Plant cell·2026
Same author

ER-phagy orchestrates virus-host interactions across kingdoms.

Trends in microbiology·2026

Related Experiment Video

Updated: Mar 18, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

26.1K

Optimizing Laguerre expansion based deconvolution methods for analysing bi-exponential fluorescence lifetime images.

Yongliang Zhang, Yu Chen, David Day-Uei Li

    Optics Express
    |July 14, 2016
    PubMed
    Summary

    This study introduces an improved least squares deconvolution method using Laguerre expansion (LSD-LE) for faster fluorescence lifetime imaging microscopy (FLIM) analysis. The enhanced method enables quicker data acquisition and broader application in FLIM-FRET studies.

    More Related Videos

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.6K
    Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
    08:31

    Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

    Published on: September 16, 2014

    12.6K

    Related Experiment Videos

    Last Updated: Mar 18, 2026

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
    09:45

    Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

    Published on: February 9, 2012

    26.1K
    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
    09:30

    Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

    Published on: January 18, 2017

    12.6K
    Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
    08:31

    Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

    Published on: September 16, 2014

    12.6K

    Area of Science:

    • Biophysics
    • Microscopy
    • Computational Imaging

    Background:

    • Accurate instrument response calibration is crucial for big fluorescence lifetime imaging microscopy (FLIM) data analysis.
    • Existing deconvolution methods can be computationally intensive, limiting acquisition speed.

    Purpose of the Study:

    • To enhance a least squares deconvolution method based on Laguerre expansion (LSD-LE) for faster and more versatile FLIM analysis.
    • To adapt the LSD-LE method for bi-exponential fluorescence decays in FLIM-Förster Resonance Energy Transfer (FRET) applications.

    Main Methods:

    • Examined a computationally effective least squares deconvolution method based on Laguerre expansion (LSD-LE).
    • Proposed new criteria for selecting Laguerre basis functions (LBFs) without mutual orthonormalities.
    • Extended the method to analyze bi-exponential fluorescence decays.

    Main Results:

    • The improved LSD-LE allows for higher laser repetition rates, reducing acquisition time.
    • The method was successfully applied to synthesized bi-exponential and realistic FLIM data.
    • Demonstrated promising results compared to previously reported constrained LSD-LE methods.

    Conclusions:

    • The enhanced LSD-LE method offers a computationally effective approach for FLIM data analysis.
    • This improved method expands the applicability of FLIM-FRET studies.
    • The technique shows potential for faster and more general FLIM image analysis.