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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 18, 2026

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
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Phasor plotting with frequency-domain flow cytometry.

Ruofan Cao, Patrick Jenkins, William Peria

    Optics Express
    |July 14, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Time-resolved flow cytometry uses phasor plots to analyze fluorescence decay from individual cells and particles. This technique effectively distinguishes cell populations based on their unique fluorescence lifetimes.

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    Area of Science:

    • Biophysics
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Interest in time-resolved flow cytometry is increasing.
    • Phasor (polar) graphics are standard in fluorescence lifetime imaging microscopy (FLIM).
    • FLIM uses phasor graphs to represent phase-shift and demodulation for image pixels.

    Purpose of the Study:

    • To adapt phasor graphics for time-resolved flow cytometry.
    • To analyze fluorescence decay kinetics in individual cells and particles.
    • To demonstrate the utility of phasor plots in discriminating cell populations.

    Main Methods:

    • Collected time-resolved flow cytometry data.
    • Integrated frequency-domain optoelectronics into a flow cytometry system.
    • Processed data to generate phasor plots where each point represents a single cell or particle.
    • Applied phasor analysis to fluorescent microspheres, Chinese hamster ovary (CHO) cells, and Saccharomyces cerevisiae cells.

    Main Results:

    • Phasor plots successfully discriminated between different populations of fluorescent microspheres and yeast cells.
    • The technique differentiated CHO cells labeled with one or two fluorophore types.
    • Phasor plots revealed distinct fluorescence lifetimes within samples.
    • Quantified cell populations based on single or dual fluorescence lifetimes.

    Conclusions:

    • Phasor plot analysis is a valuable method for time-resolved flow cytometry.
    • This approach enables the discrimination of cell populations based on fluorescence lifetime.
    • The technique facilitates the analysis of complex fluorescence decay kinetics in biological samples.