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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Related Experiment Video

Updated: Mar 18, 2026

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
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Fluorescent taggants with temporally coded signatures.

Siyang Wang, Raul Vyas, Chris Dwyer

    Optics Express
    |July 14, 2016
    PubMed
    Summary

    This study introduces fluorescent taggants with unique temporal signatures using resonance energy transfer (RET) networks. This novel approach enhances coding capacity and detection efficiency for advanced applications.

    Area of Science:

    • Photonic materials
    • Fluorescence spectroscopy
    • Biophysics

    Background:

    • Fluorescent taggants are crucial for identification and tracking.
    • Current methods face limitations in coding capacity and detection efficiency.
    • Resonance energy transfer (RET) offers a potential pathway for advanced taggant design.

    Purpose of the Study:

    • To develop novel fluorescent taggants utilizing temporally coded signatures.
    • To leverage resonance energy transfer (RET) networks for enhanced coding capacity.
    • To improve the efficiency and accuracy of fluorescent taggant detection.

    Main Methods:

    • Implementation of fluorescent taggants based on RET networks between chromophores.
    • Encoding temporal signatures as phase-type distributions determined by RET network geometry.

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  • Utilizing multinomial distribution of detected photons and Maximum Likelihood Estimation for identification.

    • Main Results:

    • Demonstrated significantly larger coding capacity compared to spectral or lifetime coded taggants.
    • Achieved high detection efficiency through time-domain signature coding.
    • Ensured high accuracy in taggant identification, even with limited photon counts and in multiplexed samples.

    Conclusions:

    • Temporally coded fluorescent taggants offer a superior alternative to existing methods.
    • The developed taggants exhibit high potential for in situ and Lidar applications.
    • This technology advances secure and efficient material identification and tracking.