Related Experiment Video
Updated: Aug 27, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
The cloning and expression of transfer RNA gene cluster of Vibrio eltor phage e4
1Indian Institute of Chemical Biology, Calcutta.
Abstract:
Vibrio eltor phage e4 codes for five different tRNA species (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 165, 606-608). The tRNA genes contained in a 3.4-kb KpnI fragment (S. Chattopadhyay and R. K. Ghosh, 1988, Virology, 162, 337-345) have been cloned in pUC 19 at the KpnI site. Two recombinant plasmids, pSR216 and pSR112, produced four of the five tRNA species (arginine, isoleucine, tyrosine, and tryptophan) encoded in the phage genome. The tRNA genes were located on a 1.45-kb KpnI-HindIII subfragment.
More Related Videos
Related Concept Videos
Bacterial Transformation
Bacterial Transformation
DNA Bacteriophages

