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Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
Laurent Holtzer1, Igor Oleinich1, Marcello Anzola1
1School of Chemistry and Biochemistry, NCCR Chemical Biology, University of Geneva , 30 quai Ernest Ansermet, Geneva, Switzerland.
Abstract:
Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.
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