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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Sensitive detection of multiple pathogens using a single DNA probe.

Noordiana Nordin1, Nor Azah Yusof2, Jaafar Abdullah2

  • 1Institute of Advanced Technology, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor, Malaysia; Food Safety Research Centre, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor, Malaysia.

Biosensors & Bioelectronics
|July 15, 2016
PubMed
Summary

This study presents a novel electrochemical DNA nanosensor for detecting food-borne pathogens. The biosensor accurately differentiates pathogens like Vibrio parahaemolyticus (VP) using specific DNA probes and gold nanoparticles.

Keywords:
Electrochemical DNA biosensorFood-borne pathogensMethylene blueNucleic acid hybridization detectionPolylactide-stabilized gold nanoparticles (PLA-AuNPs)

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Area of Science:

  • Biosensing
  • Nanotechnology
  • Food Safety

Background:

  • Food-borne pathogens pose significant health risks.
  • Accurate and rapid detection methods are crucial for public health.
  • Existing detection methods can be time-consuming or require complex equipment.

Purpose of the Study:

  • To design and construct a simple electrochemical DNA nanosensor.
  • To apply the nanosensor for differentiating food-borne pathogens.
  • To achieve sensitive and specific detection of Vibrio parahaemolyticus (VP).

Main Methods:

  • Utilized a screen-printed carbon electrode (SPCE) modified with polylactide-stabilized gold nanoparticles (PLA-AuNPs).
  • Employed methylene blue (MB) as a redox indicator for DNA hybridization detection.
  • Assessed immobilization and hybridization using differential pulse voltammetry (DPV).

Main Results:

  • The DNA nanosensor specifically distinguished complementary, non-complementary, and mismatched oligonucleotides.
  • Achieved a detection limit of 5.3×10⁻¹² M for DNA.
  • Demonstrated high stability and portability with >80% recovery after 6 months of storage.
  • Showed reliable and sensitive detection of VP with minimal cross-reactivity.

Conclusions:

  • The developed electrochemical DNA nanosensor is a promising tool for food-borne pathogen detection.
  • The sensor offers specificity, sensitivity, stability, and portability.
  • This technology can contribute to improved food safety monitoring and public health protection.